Name David Huen
Job Title Senior Lecturer in Bioinformatics
Faculty Faculty of Science and Engineering
School School of Sciences
Subject(s) Bioinformatics; Molecular Biology
Tel 01902 322154
Email d.huen@wlv.ac.uk

Biology, Chemistry and Forensic Science

David originally trained in chemical engineering, pursuing a BSc(Hons) in the subject at the University of Birmingham, followed by six years as a Technologist at the Shell Pulau Bukum refinery in Singapore.  In 1986, he decided to retrain as a molecular biologist, taking an MSc in molecular biology at the University of Sussex, then a PhD in cancer studies at the University of Birmingham on Epstein-Barr virus-mediated growth transformation.  He continued in EBV research on a Beit ZZM fellowship before further postdoctoral research at Cambridge where he diversified into Drosophila genetics and bioinformatics.

He joined the University of Wolverhampton in 2012 and is now Senior Lecturer in Bioinformatics.  He currently provides teaching in molecular biology and bioinformatics.

He is currently module leader for:-

6AB015 (Advanced biology practicals)

5BC001 (Molecular biosciences)

6BC002 (Gene manipulation and bioinformatics)

6BC004 (Genetics and genomics in pharmacy)

7BC006 (Contemporary applied bioinformatics)

and also teaches on:-

5BC003 (Molecular biosciences practical techniques)

6BC001(Advanced topics in molecular biosciences)

6PY002 (Pharmaceutical biotechnology and molecular biology)

6FS006 (Honours projects)

7AB002 (Masters lab techniques)

7BC002 (Molecular genetics and genomics)

7BC003 (DNA data mining)

Bioinformatics; molecular biology; gene drive systems; machine learning; metagenomics

Metagenomics concerns itself with the genomics populations containing significant numbers of unculturable microbes.  Instead of analysing the sequences from axenic cultures, metagenomic analysis infers the constituent genomes by analysing sequences directly obtained from mixed populations.  David is interested in the development and application of algorithms for metagenomic analysis.  He has latterly performed an analysis of the antibiotic resistance genes present in the human microbiome and 16S analysis of stool microbiota from Canada geese (Branta canadensis).

He is also interested more generally in the application of computational techniques to the analysis of biological phenomena.  Currently, he has been investigating the use of machine learning to large-scale protein classification.  He also has collaborations where he has supplied bioinformatics support to projects involving somatic mutation detection within tumor-normal pairs and identification of genes involved in chemotherapeutic drug resistance using the near-haploid KBM7 cell line.

  • Associate member of the Institute of Chemical Engineers
  • Fellow of the Higher Education Academy
  • Pangeni RP, Channathodiyil P, Huen DS, Eagles LW, Johal BK, Pasha D, Hadjistephanou N, Nevell O, Davies CL, Adewumi AI, Khanom H, Samra IS, Buzatto VC, Chandrasekaran P, Shinawi T, Dawson TP, Ashton KM, Davis C, Brodbelt AR, Jenkinson MD, Bieche I, Latif F, Darling JL, Warr TJ, Morris MR (2015) The GALNT9, BNC1 and CCDC8 genes are frequently epigenetically dysregulated in breast tumours that metastasise to the brain. Clin Epigenetics 7:57.
  • Russell C, Begum S, Hussain Y, Hussain M, Huen D, Rahman AS, Perrie Y, Mohammed AR (2015) Pediatric drug development of Ramipril: reformulation, in viro and in vivo evaluation. J Drug Targeting doi://10.3109/1061186X.2015.1036275
  • Simoni A, Siniscalchi C, Huen D, Chan YS, Russell S, Windbichler N, Crisanti A (2014) Development of Synthetic Selfish Elements Based on Modular Nucleases in Drosophila melanogaster.   Nucleic Acids Res. 42:461-7472.
  • Chan Y-S, Takeuchi R, Jarjour J, Huen DS, Stoddard BL, Russell S (2013) The design and in vivo evaluation of engineered I-OnuI-based enzymes for HEG gene drive. PLoS ONE 8(9): e74254.
  • Chan Y-S, Huen DS, Glauert R, Whiteway E, Russell S (2013) Optimising homing endonuclease gene drive performance in a semi-refractory species: The Drosophila melanogaster experience. PLoS ONE 8(1):e54130. 
  • Chan, YS, Naujoks, DA, Huen, DS, Russell, S (2011) Insect population control by homing endonuclease-based gene drive: an evaluation in Drosophila melanogaster.  Genetics 188:33-44
  • Huen DS, Russell, S (2010) On the use of resampling tests for evaluating statistical significance of binding-site co-occurrence. BMC Bioinformatics 11:359.
  • Yan J, Huen D, Morley T, Johnson G, Gubb D, Roote J, and Adler PN (2008) The multiple-wing-hairs gene encodes a novel GBD–FH3 Domain-containing protein that functions both prior to and after wing hair initiation. Genetics 180:219-228.
  • Holland RCG, Down TA, Pocock M, Prlic A, Huen D, James K, Foisy S, Dräger A, Yates A, Heuer M, and Schreiber MJ (2008) BioJava: an open-source framework for bioinformatics. Bioinformatics 24: 2096-2097.
  • Birch-Machin I, Gao S, Huen D, McGirr R, White RAH, Russell S (2005) Genomic analysis of heat-shock targets in Drosophila. Genome Biol. 2005;6(7):R63.
  • Ryder E, Blows F, Ashburner M, Bautista-Llacer R, Coulson D, Drummond J, Webster J, Gubb D, Gunton N, Johnson G, O'Kane CJ,Huen D, Sharma P, Asztalos Z, Baisch H, Schulze J, Kube M, Kittlaus K, Reuter G, Maroy P, Szidonya J, Rasmuson-Lestander A, Ekstrom K, Dickson B, Hugentobler C, Stocker H, Hafen E, Lepesant JA, Pflugfelder G, Heisenberg M, Mechler B, Serras F, Corominas M, Schneuwly S, Preat T, Roote J, Russell S (2004) The DrosDel collection: A set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics 167 (2): 797-813.
  • Frolov MV, Huen DS, Stevaux O, Dimova D, Balczarek-Strang K, Elsdon M, Dyson NJ (2001) Functional antagonism between E2F family members. Genes. Dev. 15 (16): 2146-2160.
  • Huen DS, Elsdon M, Ponder BAJ (2000) The Drosophila Ret gene is transcribed in multiple alternatively spliced forms. Mol. Gen. Genet. 264 (3): 335-340.
  • Gubb D, Green C, Huen D, Coulson D, Johnson G, Tree D, Collier S, Roote J (1999) The balance between isoforms of the Prickle LIM domain protein is critical for planar polarity in Drosophila imaginal discs. Genes. Dev.  13 (17): 2315-2327.
  • McCarthy NJ, Hazlewood SA, Huen DS, Rickinson AB, Williams GT (1996) The Epstein-Barr virus gene BHRF1, a homologue of the cellular oncogene Bcl-2, inhibits apoptosis induced by gamma radiation and chemotherapeutic drugs. Adv Exp Med Biol 406:83-97.
  • Huen DS, Henderson SA, Croom-Carter D, Rowe M (1995) The Epstein-Barr-virus latent membrane protein-1 (LMP1) mediates activation of NF-kB and cell-surface phenotype via 2 effector regions in its carboxy-terminal cytoplasmic domain. Oncogene 10 (3): 549-560 .
  • Henderson SA, Huen D, Rowe M (1994) Epstein-Barr-virus transforming proteins Semin. Virol. 5 (5): 391-399.
  • Rowe M, Peng-Pilon M, Huen DS, Hardy R, Croom-Carter D, Lundgren E, Rickinson AB (1994) Up-regulation of bcl-2 by the Epstein-Barr virus latent membrane-protein LMP1 - a B-cell-specific response that is delayed relative to NF-kB activation and to induction of cell-surface markers. J. Virol. 68 (9): 5602-5612.
  • Henderson S, Huen D, Rowe M, Dawson C, Johnson G, Rickinson A (1993)  Epstein-Barr virus-coded BHRF1 protein, a viral homolog of BCL-2, protects human B-cells from programmed cell death.  Proc. Natl. Acad. Sci. USA 90:8479-8483.
  • Takayama S, Cazals-Hatem DL, Kitada S, Tanaka S, Miyashita T, Hovey LR, Huen D, Rickinson A, Veerapandian P, Krajewski S, Saito K, Reed JC (1994) Evolutionary conservation of function among mammalian, avian, and viral homologs of the bcl-2 oncoprotein.DNA Cell Biol. 13 (7): 679-692
  • Huen DS, Fox A, Kumar P, Searle PF (1993) Dilated heart-failure in transgenic mice expressing the Epstein-Barr virus nuclear antigen-leader protein.J. Gen. Virol. 74: 1381-1391.
  • Grand RJA, Byrd PJ, Grabham PW, Gregory CD, Huen DS, Merrick RM, Young LS, Gallimore PH (1989) The expression of the retinoblastoma gene-product RB1 in primary and adenovirus-transformed human-cells. Oncogene 4 (11): 1291-1298.

See http://www.researcherid.com/rid/F-3676-2014